Temporal Resolution in Electrochemical Imaging on Single PC12 Cells Using Amperometry and Voltammetry at Microelectrode Arrays

Detection of exocytosis at individual pancreatic beta cells by amperometry at a chemically modified microelectrode.

Amperometry at a carbon fiber microelectrode modified with a composite of ruthenium oxide and cyanoruthenate was used to monitor chemical secretions of single pancreatic beta cells from rats and humans. When the insulin secretagogues glucose, tolbutamide, and K+ were applied to the cell, a series of randomly occurring current spikes was observed. The current spikes were shown to be due to the d...

Snapshot voltammetry using a triangular bipolar microelectrode.

In this paper, we report a new electroanalytical technique we call snapshot voltammetry. This method combines the properties of bipolar electrodes with electrogenerated chemiluminescence (ECL) to provide a means for recording optical voltammograms in a single micrograph. In essence, the information in a snapshot voltammogram is contained in the spatial domain rather than in the time domain, whi...

Creation of Defined Single Cell Resolution Neuronal Circuits on Microelectrode Arrays

DNA-barcode directed capture and electrochemical metabolic analysis of single mammalian cells on a microelectrode array.

A microdevice is developed for DNA-barcode directed capture of single cells on an array of pH-sensitive microelectrodes for metabolic analysis. Cells are modified with membrane-bound single-stranded DNA, and specific single-cell capture is directed by the complementary strand bound in the sensor area of the iridium oxide pH microelectrodes within a microfluidic channel. This bifunctional microe...

Microelectrode Arrays

The development of implantable devices which enable fully wireless transmission of brain signals, recorded by microelectrode arrays (MEA) as dynamical neural circuit information, is one important goal for advancing human neuroprostheses. Current brain sensing research for prostheses via first clinical trials is offering tantalizing glimpses for the future, even if these technologies are cumbers...